(Answered)-The molar extinction coefficient of a solution of concentration - (2025 Updated Original AI-Free Solution

Question

1. The molar extinction coefficient of a solution of concentration 0.04 M and thickness 10.5 cm is 80.08 L mol^-1m^-1. Find the value of ? ? ? ? ? ? ? optical density.
2. Absorbance of a solution is found to be 0.83 .Calculate % Transmittance.
3. When a wavelength of 250 nm is passed through a solution, the light intensity is reduced to 20% of its initial value. Then absorbance is ? ? given by _____
4. The absorbance of 1 mm of a sample solution whose concentration is 0.03 M is _____. (Given that the molar absorption coefficient is 10 ? L mol^-1m^-1)
5. An unknown solution showed absorbance 5.000 and molar absorption coefficient, ?= 160 L mol^-1m^-1, (Thickness, l=2 cm). Calculate ? ? ? ? the concentration of the solution.

1. Analyse the protein content in different samples using the above constructed protein standard curve.
2. Find out the specific activity of beta amylase enzyme.
3. If 1ml of an unknown sample of protein is diluted to 10ml, and a? 0.5ml portion of the diluted sample is found to contain 50ug of protein when assayed for protein using the Lowry method. What is the concentration of protein in ug/ml in the original sample ?
4. If you mix two? unknown samples and repeat the Lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is used?
5. Based on the experimental data provided, estimate the amount of reducing sugar in the given unknown solution by Dinitrosalicylate [DNS] method. ???

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Analyse the protein content in different samples using the above constructed protein standard curve.Find out the specific activity of beta amylase enzyme .?If 1ml of an unknown sample of protein is diluted to 10ml, and a? 0.5ml portion of the diluted sample is found to contain 50ug of protein???? when assayed for protein using the lowry method. What is the concentration of protein in ug/ml in the original sample ?If you mix two? unknown samples and repeat the lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is? used

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